MCLAB/T4 RNA连接酶2（截短）/T4RL2T-200/20000U-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/T4 RNA连接酶2（截短）/T4RL2T-200/20000U

MCLAB/T4 RNA连接酶2（截短）/T4RL2T-200/20000U

商品编号： T4RL2T-200

品牌： Molecular Cloning Laboratories

市场价： ¥8160.00

美元价： 4896.00

产地：美国(厂家直采)

公司：

产品分类：连接酶

公司分类： ligase

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

MCLAB’struncated T4RNALigase2wasdevelopedspecificallyfordemandingNext-GenerationRNASequencingapplications. Thetruncated ligase2specificallyligatestheadenylated5´endofanadaptertothe3´endofRNA.TheenzymedoesnotrequireATPforligationbutdoesneedanadenylatedsubstrate.BynothavingextraATPinthereactionitwilldramaticallyreducetheamountofligationbetweenrandomRNAmolecules.

Description:
MCLAB’struncatedT4RNALigase2wasdevelopedspecificallyfordemandingNext-GenerationRNASequencingapplications.Thetruncatedligase2specificallyligatestheadenylated5'-endofanadaptertothe3'-endofRNA.TheenzymedoesnotrequireATPforligationbutdoesneedanadenylatedsubstrate.BynothavingextraATPinthereaction,theamountofligationbetweenrandomRNAmoleculesisdramaticallyreduced.UnlikethefulllengthT4RNAligase2,thetruncatedligasedoesnotligatethephosphorylated5'-endofRNAorDNAwithouttheadenylatedsubstrate,makingitanexcellentchoiceforsmallRNAlibrarypreparation.WhetheryourplantosequencemiRNAsortoperformdirectionalmRNA-Seq,thetruncatedligase2willhelptoenhanceyourlibrarypreparations.

T4Rnaligase2dsrna.jpg"
Figure,BioanalyzertraceofligationproductwithT4RNALigase2(dsRNALigase).Theenzymecanefficientlyligate3'OHofRNAfragmenttothe5'phosphateofDNAfragmentinadoublestrandedstructure.Redcolortrace:Newlotenzymereaction;Bluecolortrace:Previouslotenzymereaction(shelflifewasnear12months).

Features:
-DevelopedforNext-GenerationRNASequencingapplications
-Efficiencyofligationatnearly100%
-IncreaseABIlitytoidentifymiRNAs

Source:
PurifiedfromanE.colistraincarryingtruncatedT4RNALigase2(1-249)overproducingplasmid.

HeatInactivation:65°Cfor20minutes

ReactionConditions:
1xT4RNALigaseReactionBuffer
Incubateat25°C

1xT4RNALigaseReactionBuffer:
50mMTris-HCl
10mMMgCl₂
1mMDTT
pH7.5@25°C

StorageConditions:
10mMTris-HCl(pH7.5)
100mMNaCl
0.1mMEDTA
0.1mMDTTand50%Glycerol

UnitDefinition:
Oneunitisdefinedastheamountoftheenzymerequiredtogive50%ligationofa17-meradenylatedoligonucleotidetoapurifiedcontrolRNAtemplatein30minutesat37°C.

SpecificActivity:1,200U/μg

RecommendedStorageCondition:-20°C

QualityControl:
Enzymepreparationsareroutinelyassessedforrelativepurity,activityandabsenceofRNaseandDNase

References:
1.Jayaprakash,A.D.,JabadoO.,Brown,B.D.andSachidanandam,R.(Sept2,2011),NucAcidRes,1–12.
2.Ho,C.K.etal.(2004)Structure,12,327-339.
3.Ho,C.K.andShuman,S.(2002)Proc.Natl.Acad.Sci.USA,99,12709-12714.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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