Description: T7DNALigasecatalyzestheformationofaphosphodiesterbondbetweena5'-phosphateanda3'-hydroxylterminiinduplexDNA.Theenzymewilljoinbluntendsandcohesiveendsterminiaswellasrepairsingle-strandednicksinduplexDNA.
Application: -JoiningofOkazakifragmentsduringreplication -Completingshort-patchDNAsynthesisoccurringinDNArepairprocess
Source: ArecombinantE.colistraincarryingtheT7DNALigasegene.
Suppliedin: 20mMTris-HCl 300mMNaCl 1mMDTT 0.1mMEDTA 50%Glycerol pH7.5@25°C
10xRapidLigationBuffer: 660mMTris-HCl 100mMMgCl2 10mMDTT 10mMATP 75%PEG6000 pH7.6@25°C UnitDefinition: OneWeissunitoftheenzymecatalyzestheconversionof1nmolof[32PPi]intoNorit-adsorbableformin20minat37°C. Enzymeactivityisassayedinthefollowingmixture:66mMTris-HCl(pH7.6),6.6mMMgCl2,0.066mMATP,10mMDTT,3.3µM[32PPi]. OneWeissUnitisapproximatelyequivalentto67cohesiveendunits(CEU).Or1000CEU=15WeissUnit. SpecificActivity: 45WeissU/µg RecommendedStorageCondition:-20°C
Reference: Doherty,A.etal.J.Biol.Chem(1996)V.271,No.19,11083-11089.
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