Description: APE1,alsoknownasHAP1orRef1,actsasanAPlyasebyhydrolyzingthephosphodiesterbackboneatthe5'-endofanapurinic(AP)site,generatinga1basegapintheDNAduplexandleaving3'-hydroxyland5'-deoxyribosephosphatetermini.EvidencesuggeststhatAPE1mayexhibitweakDNA3'-diesterase,3'to5'-exonucleaseandRNaseHactivities(1-4).
SpecificActivity:2,000,000U/mg
Application: -Singlecellgelelectrophoresis(Cometassay) -Alkalineelution -Alkalineunwinding -Modifiednicktranslation
Source: AnE.colistrainwhichcarriestheclonedhumanAPE1gene.
Suppliedin: 10mMTris-HCl 50mMNaCl 1mMDithiothreitol 0.1mMEDTA 50%Glycerol pH8.0@25°C
Suppliedwith: 10xGreenBuffer
10xGreenBuffer: 200mMTris-Acetate 500mMPotassiumAcetate 10mMDithiothreitol 100mMMagnesiumAcetate pH7.9@25°C
UnitDefinition: Oneunitisdefinedastheamountofenzymerequiredtocleave20pmolofa34-meroligonucleotideduplexcontain-ingasingleAPsitein1hourat37°C.
RecommendedStorageCondition:-20ºC
Reference: 1.Demple,B.etal.(1991)Proc.Natl.Acad.Sci.USA,88,11450-11454. 2.Barzilay,G.etal.(1995)Nucl.AcidsRes.,23,1544-1550. 3.Barzilay,G.etal.(1995)NatureStruc.Biol.,2,451-468. 4.Unpublishedobservations(See“Notes”Section) |