Description: Fpg(alsoknownasFormamidopyrimidineDNAglycosylase,MutM,FAPYDNAGlycosylase,and8-oxoguanineDNAglycosylase)participatesinthebase-excision(BER)pathwayofDNArepairenzymesandactsbothasaN-glycosylaseandanAP-lyase.TheN-glycosylaseactivityreleasesdamagedpurinesfromdoublestrandedDNA,generatinganapurinic/apyrimidinic(APsite).TheAP-lyaseactivitycleavesboththe3′and5′phosphodiesterbondsattheAPsite,producinga1basegapintheDNAand3′and5′phosphatetermini.BasesrecognizedandremovedbyFpginclude7,8-dihydro-8-oxoguanine(8-oxoguanine),8-oxoadenine,fapy-guanine,methy-fapy-guanine,fapy-adenine,aflatoxinB1-fapy-guanine,5-hydroxy-cytosineand5-hydroxy-uracil(1,2).
Application: -DNANicking -DNARepair -NickTranslation
Source: ArecombinantE.colistraincarryingtheclonedfpggene.
SpecificActivity:20,513U/mg
SuppliedWith: 10XFpgBuffer Incubateat37°C
10XFpgBuffer: 100mMBis-Tris-Propane 100mMMgCl2 10mMDTT 1mg/mlBSA pH7.0@25°C
Suppliedin: 20mMTris-HCl 50mMNaCl 1.0mMDTT 0.1mMEDTA 50%glycerol pH8.0@25°C
UnitDefinition: Oneunitisdefinedastheamountofenzymerequiredtocleave1pmolofa34meroligo-nucleotideduplexcontainingan8-oxoguaninebasepairedwithacytosinein1hourat37°Cinatotalreactionvolumeof10µlinreactionbuffer.
RecommendedStorageCondition:-20°C
References: 1.Tchou,J.etal.(1994)SubstratespecificityofFpgprotein.J.Biol.Chem.,269,15318-15324. 2.Hatahet,Z.etal.(1994)Newsubstratesforoldenzymes.J.Biol.Chem.,269,18814-18820. |