MCLAB/Cas9核酸酶（wt）/Cas9-200/1 nmol，50µl-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/Cas9核酸酶（wt）/Cas9-200/1 nmol，50µl

MCLAB/Cas9核酸酶（wt）/Cas9-200/1 nmol，50µl

商品编号： CAS9-200

品牌： Molecular Cloning Laboratories

市场价： ¥5000.00

美元价： 3000.00

产地：美国(厂家直采)

公司：

产品分类：寡聚核酸

公司分类： Oligomeric_nuclei_acid

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Cas9Nuclease,Streptococcuspyogenes,isanRNA-guidedendonucleasethatcatalyzessite-specificcleavageofdoublestrandedDNA.

Introduction:
Cas9(CRISPRassociatedprotein9)isaRNA-guidedDNAendonucleaseenzymeassociatedwiththeCRISPR(ClusteredRegularlyInterspacedShortPalindromicRepeats)adaptiveimmunitysystemin Streptococcuspyogenes.TheCRISPR/Cas9systemisthelatestRNA-guided,endonucleasetoolingenomeeditingwhichallowsforveryspecificgenomicdisruptionandreplacement.Guidedbyatarget-specific,singleguideRNA(sgRNA),theCas9nucleaseproteinservestounwindthegenomicDNAduplexandcleavebothstrandsuponrecognitionofthetargetsequencebythesgRNA,resultingindouble-strandedbreaks.Theresultingdouble-strandedbreakgetsrepairedbythenon-homologousendjoining(NHEJ)pathway,leADIngtoadisruptionintheopenreadingframeofthetargetedgene.

CRISPR-Cas9 System

Description:
RecombinantCas9-NLScomprisestheentireCas9proteinsequence(1368aminoacids)fusedtoaproprietarynuclearlocalizationsequence(NLS)anda6XHistagattheC-terminus.Theproteinhasanapparentmolecularweightof162kDaonSDS-PAGE.

Cas9variantsforvariousgenomemanipulationsareavailablein8forms:

Cas9(WT)
Cas9withnuclearlocalizationsignal(NLS)
Cas9with3NLS(onC-terminus)forbetternuclearlocalization
NickaseCas9(D10A)fornicking
dCas9(D10A/H840A)forsimplebinding
Hi-Fi1Cas93NLS(K848A,K1003A,R1060A)andHi-Fi2Cas93NLS(N497A,R661A,Q695A,Q926A)forreducinggenome-wideoff-targeteffects
dCas9-biotinylatedforimmunoprecipitation

Features:
LowendotoxinLevel
HighBIOLOGicalactivity
Consistentfromlottolot

Application:
Genomeediting

Source:
Cas9genefromStreptococcuspyogenesexpressedinE.coli

Purity:
Greaterthan97%asdeterminedbySDS-PAGE.

Activity:
96%ofPCRproductdigestionafter30minutesat37°C.Thereactionisperformedasfollow;20µlreaction,including100nMCas9protein,200nMtracrRNA,200nMEMX1-specificcrRNA,200ngEMX1-specificPCRproductand1xdigestionbuffer(20mMHEPES,100mMNaCland5mMMgCl2,pH6.5).Incubate30minutesat37°C,andstopreactionwith1µlRNAseA(1mg/ml).

Storagecondition:-20°C

10XCas9ReactionBuffer:
200mMHEPES,50mMMgCl2,1MNaCl,1mMEDTA,pH6.5

StorageBuffer:
50mMTris-HCl
50MKCl
1mMDTT
1mMEDTA
50%Glycerol
pH7.5

ExperimentalData

References

JinekM,ChylinskiK,FonfaraI,HauerM,DoudnaJA,CharpentierE.(2012)Aprogrammabledual-RNA-guidedDNAendonucleaseinadaptivebacterialimmunity.Science.Aug17;337(6096):816-21.
MaliP,YangL,EsveltKM,AachJ,GuellM,DiCarloJE,NorvilleJE,ChurchGM.(2013)RNA-guidedhumangenomeengineeringviaCas9.Science.Feb15;339(6121):823-6.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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PCR试剂荧光素酶嗜热DNA聚合酶蛋白质纯化液逆转录酶和RNA聚合酶蛋白质G NGS库准备工具包 PCR试剂盒 DNA修复蛋白磁性微球聚合酶链反应聚合酶链反应 PCR引物组激酶类 DNA提取培养皿核酸酶蛋白质阵列阻滞剂伴侣蛋白与二硫键异构酶伴侣蛋白与二硫键异构酶生化试剂试剂盒 DNA梯蛋白质纯化树脂重组蛋白嗜中温DNA聚合酶磷酸酶和磺酰化酶螺旋酶溶菌酶蛋白酶 CRISPR/Cas9目标检测单链DNA结合蛋白 NGS库定量试剂盒 NGS杂项质粒制备试剂盒碎片分析主管细胞实时PCR试剂盒 DNA/RNA连接酶附件和试剂限制性内切酶核糖核酸酶

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