Description:
ExonucleaseIII(E.coli)isa3'->5'exonucleasewhichactsbydigestingonestrandofadsDNAduplexatatimeordigestingtheRNAstrandofanRNA-DNAheteroduplex^(1,2).ExonucleaseIII(E.coli)breaksphosphodiesterbondsonthe5'-sideofAPsitesinbothdsDNAandssDNA⁽³⁾.Itremoves3'-terminalgroupsondsDNA⁽³⁾,increasesMutYturnover⁽⁴⁾,andefficientlydegrades3'-recessedbutnot3'protrudingDNAends(creating5'-overhangs)⁽⁵⁾.ExonucleaseIII(E.coli)removesalimitednumberofnucleotidesperbindingevent,resultingincoordinatedprogressivedeletionswithinthepopulationofDNAmolecules⁽¹⁾.

SpecificActivity:150,000U/mg

Application:
Degradesexcesssingle-strandedprimeroligonucleotidesfromareactionmixturecontainingdouble-strandedextensionproducts.

Source:
PurifiedfromastrainofE.colithatexpressestherecombinantExonucleaseIIIgene.

Suppliedin:
25mMTris-HCl
50mMKCl
1.0mMDTT
0.1%mMEDTA
50%Glycerol
pH8.0@25°C

Suppliedwith:10xYellowBuffer

10xYellowBuffer:
100mMBis-Tris-Propane
100mMMgCl₂
10mMDTT
pH7.0@25°C

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtoproduce1nmolofacid-solubletotalnucleotidein30minutesat37°C.

RecommendedStorageCondition:-20°C

References:
1.Linn,S.M.(1982)Nucleases,pp.291-309,ColdSpringHarborLaboratoryPress.
2.Shida,T.,etal.(1996)Nucl.AcidsRes.24(22),4572-4576.
3.Doetsch,P.W.(1990)Mutat.Res.236(2-3),173-201.
4.Pope,M.A.,etal.(2002)J.Biol.Chem.277(25),22605-22615.
5.Henikoff,S.(1984)Gene28,351-359.