Description: ExonucleaseIcleavessingle-strandedDNAinthe3'->5'direction,releasing5'-mono/di-nucleotidesandleavingdouble-strandedDNAmoleculesandthe5'-terminusintact.Theenzymeisprocessedthroughdigestionandisinhibitedbythepresenceofa3'-terminalphosphate.ExonucleaseIistolerantofawide-rangeofbufferconditionsandcantypicallybeaddedtoreactionscontainingmagnesium(1-3).
SpecificActivity:185,000U/mg
Application: -RemovalofresidualssDNA,includingoligos,fromreactionmixes
Source: PurifiedfromastrainofE.colithatexpressestherecombinantExonucleaseIgene.
Suppliedin: 10mMTris-HCl 100mMNaCl 1mMDTT 0.5mMEDTA 50%Glycerol pH7.5@25°C 10xExonucleaseIBuffer 670mMGlycine-KOH 67mMMgCl2 100mM2-mercaptoethanol (pH9.5@25°C) UnitDefinition: Oneunitisdefinedastheamountofenzymerequiredtoproduce10nmolofacid-solubletotalnucleotidein30minutesat37°C.
RecommendedStorageCondition:-20°C
Reference: 1.Lehman,I.R.andNussbaum,A.L.(1964)J.Biol.Chem.239,2628. 2.Kushner,S.R.etal.(1971)Proc.Natl.Acad.Sci.USA68,824. 3.Kushner,S.R.etal.(1972)Proc.Natl.Acad.Sci.USA69,1366. |