Description: Inorganicpyrophosphatase(PPase)isubiquitousinnatureandplaysanimportantroleinenergymetabolism,providingathermodynamicpullforbiosyntheticreactionssuchasprotein,RNA,andDNAsynthesis.EscherichiacoliK-12geneppaencodinginorganicpyrophosphatase(PPase)wasclonedandsequenced.The5'-endoftheppamRNAwasidentifiedbyprimerextensionmapping.
Source: E.colistraincarryingaplasmidencodingpyrophosphatasefromE.coliK-12.
Application: -Roleinprotein,RNA,andDNAsynthesis -CatalyzingthereactionPPi+H2O—>2Pi
UnitDefinition: Oneunitwillrelease1.0µmoleofinorganicorthophosphateperminuteatpH8.5at37°C. SpecificActivity: 3,500U/mg
RecommendedStorageCondition:-20°C
Reference: Kornberg,A.1962.Onthemetabolicsignificanceofphosphorolyticandpyrophosphorolyticreactions,p.251-264.InH.KashaandP.Pullman(ed.),Horizonsinbiochemistry.AcademicPress,NewYork. |