Recognitionsite: GGTCTC(N)1Λ CCAGAG(N)5
Source: An E.coli strainthatcarriestheBsaIgenefrom BacillusstearoThermophilus UnitDefinition Oneunitisdefinedastheamountofenzymerequiredtodigest1µgofpXbaDNAin1hourat37°Cinatotalreactionvolumeof50 µl. ReactionCondition 1XBsaIBufferat37°C 10xBsaIBuffer: 100mMTris-HCl(pH8.5at37°C) 100mMMgCl2 1MKCl 1mg/mlBSA
StorageBuffer: 10mMTris-HCl 300mMNaCl 1mMDTT 0.1mMEDTA 500μg/mlBSA 50%Glycerol pH7.4@25°C
HeatInactivation 65°Cfor20min Typicalligation/recutassayresult: Morethan95%oftheDNAfragmentscanbeligatedaftera10-foldover-digestionofpXbaDNAwithBsaI,andmorethan95%oftheseDNAfragmentscanberecutwithBsaI. Recommendedstoragecondition: -20°C Comments: Blockedbyoverlapping dcm methylation.CleavageofmammaliangenomicDNAisblockedbysomecombinationsofoverlappingCpGmethylation.Activityat50°Cis100%. |