MCLAB/HoTaq DNA聚合酶（热启动）/HT-210/5000单位，5 U/#956；L-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/HoTaq DNA聚合酶（热启动）/HT-210/5000单位，5 U/#956；L

MCLAB/HoTaq DNA聚合酶（热启动）/HT-210/5000单位，5 U/#956；L

商品编号： HT-210

品牌： Molecular Cloning Laboratories

市场价： ¥14800.00

美元价： 8880.00

产地：美国(厂家直采)

公司：

产品分类： DNA聚合酶

公司分类： DNA_polymerase

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

HoTaq™DNAPolymeraseisahot-startTaqDNAPolymerase,whichischemicallymodifiedformTaqDNAPolymerase.

Description:
HoTaq™DNAPolymeraseisahot-startTaqDNAPolymerase,whichisachemically modifiedformofTaqDNAPolymerase.HoTaqDNAPolymeraseisprovidedinaninactivestateandhasaminimumenzymaticactivityatambienttemperatures.Itwillbecomeactiveafter10minutesheatingat95ºC.Thispreventstheformationofmisprimedproductsduringreactionsetupandthefirstdenaturationstep,leadstohighPCRspecificity.Itissuitablefordiagnosticreactionwithouttheminerband.Theenzymeisahighprocessive5'->3'DNApolymerasethatlacks3'->5'exonucleaseactivity.EachlotofHoTaqDNApolymeraseistestedforPCRamplification.

Suppliedwith:
10xTaqPCRBuffer(NodNTP)

Suppliedin:
20mMTris-HCl(pH8.0)
100mMKCl
0.5%Tween20
0.1mMEDTA
1mMDTT
50%(v/v)glycerol

Comparison:
HereistheresultofcomparingMCLAB'sHoTaqwithotherleADIngbrands.

MCLabHoTaqQuality.jpg"

Source:
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticus(sameasTaqDNAPolymerase).

RecommendedStorageCondition:-20°C

RecommendedReactionConditions:
95°C,10minutes.->(95°C,10seconds.->55°C,30seconds.->72°C,30seconds.)for25cycles.

UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPsintoacid-insolublematerialin30minutesat75°C.

References:
1.ChienA,EdgarDB,TrelaJM(1976)."DeoxyribonucleicacidpolymerasefromtheextremeThermophileThermusaquaticus".J.Bact.127(3):1550–7.PMC232952.PMID8432.
2.Saiki,RK;etal.(1988)."Primer-directedenzymaticamplificationofDNAwithathermostableDNApolymerase.".Science239(4839):487–91.doi:10.1126/science.2448875.PMID2448875.
3.Saiki,RK;etal.(1985)."Enzymaticamplificationofbeta-globingenomicsequencesandrestrictionsiteanalysisfordiagnosisofsicklecellanemia".Science230(4732):1350–4.doi:10.1126/science.2999980.PMID2999980.
3.LawyerFC,etal.(1993)."High-levelexpression,purification,andenzymaticcharacterizationoffull-lengthThermusaquaticusDNApolymerase...".PCRMethodsAppl.2(4):275–87.PMID8324500.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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服务热线：4000-520-616

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手机：18915418616

官网：http://www.mclab.com/