GeneralDescription I-5Hi-FiDNApolymeraseisanultra-fastandhigh-fidelityDNApolymerase.Itprovidesrobustamplificationoffromdifferenttemplatesincludingplasmids,BACS,genomicDNA,andlambdaDNA.Itallowsforamplificationofupto8kbwithhumangenomicDNAandupto21kbwithlambdaDNA.Ithasanextensionspeedof1kb/10-15secondsdependingontemplatetype.ThisallowstheusertosavetimebyspeedingupPCRreactionsandprovideshigherfidelitythanTaqorPfu.
Feature •Robust–maximalsuccessandminimaloptimizationneeded •Fidelity–50XgreaterthanTaq •HighSpeed–10XfasterthanPfu •HighYield •Versatile–bestforlongordifficulttemplates
Source E.coli Applications PCR Cloning LongorDifficultAmplification High-ThroughputPCR Suppliedwith 5XReactionBuffer(1ml),50mMMgCl2(1ml) Suppliedin(bufferdescription) 20mMTris-HCl,100mMKCl,1mMDTT,0.1mMEDTA,200ug/mlBSA,50%glycerol,1XstABIlizer,pH7.4@25C StorageCondition -20°C UnitDefinition Oneunitistheamountofenzymethatincorporates5nmolofdNTPsintoacidinsolublematerialin15minutesat72°C. References Frey,B.andSuppman,B.(1995).BioChemica.2,34-35. Chester,N.andMarshak,D.R.(1993).AnalyticalBiochemistry.209,284-290.
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