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当前位置: 首页 > 产品中心 > DNA_polymerase > MCLAB/I-5热启动DNA聚合酶/I5HD-100/1 Ea
商品详细MCLAB/I-5热启动DNA聚合酶/I5HD-100/1 Ea
MCLAB/I-5热启动DNA聚合酶/I5HD-100/1 Ea
MCLAB/I-5热启动DNA聚合酶/I5HD-100/1 Ea
商品编号: I5HD-100
市场价: ¥2950.00
美元价: 1770.00
产地: 美国(厂家直采)
公司:
产品分类: DNA聚合酶
公司分类: DNA_polymerase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

I-5Hi-FiDNAHotStartPolymeraseisahigh-fidelityand fastDNApolymerase.

GeneralDescription
I-5Hi-FiDNAHotStartPolymeraseisanultra-fastandhigh-fidelityDNApolymerase.Itprovidesrobustamplificationofdifficulttemplatesincludingplasmids,BACS,genomicDNA,andlamBDaDNA.Itallowstheamplificationofupto8kbhumangenomicDNAandupto21kblambdaDNA.Ithasanextensionspeedof1kb/10-15secondsdependingontemplatetype.ThisallowsuserstosavetimebyspeedingupPCRreactionsandprovideshigherfidelitythanTaqorPfu.TheenzymecontainsaHotStartmechanismthatinactivatestheenzymeuntilitisheated.ThisallowsuserstosetupPCRreactionsatroomtemperaturewithoutworryingaboutprimerdimersornon-specificpreamplification.

Feature
•Fast–4XfasterthanhotstartTaq
•Robust–highinhibitortolerance
•Highyields–highefficiency
•LongPCRproducts

Source
E.coli

Applications
HotStartPCR
RoutinePCR
FastPCR
HighthroughputPCR
Genotyping

Suppliedwith
5XReactionBuffer(1ml,5XMgClincluded )

50mMMgCl2(1ml)(foroptimization)

Suppliedin(bufferdescription)
20mMTris-HCl,100mMKCl,1mMDTT,0.1mMEDTA,200ug/mlBSA,50%glycerol,1XstABIlizer,pH8.0@25C

StorageCondition
-20ºC

UnitDefinition
Oneunitistheamountofenzymethatincorporates5nmolofdNTPsintoacidinsolublematerialin15minutesat72°C.

Reference
Frey,B.andSuppman,B.(1995).BioChemica.2,34-35.
Chester,N.andMarshak,D.R.(1993).AnalyticalBiochemistry.209,284-290.

ShippingCondition
dryice

Userprotocol

 

 

品牌介绍
MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.
 25µlReaction50µlReactionFinalConcentration
    
Instructions   
    
 25µlReaction50µlReactionFinalConcentration
I-55XBuffer5ul10ul1X(seenotes)
10µMPrimerA1µl2µl400µM
10µMPrimerB1µl2µl400µM
TemplateDNAasneededasneededseenotes
50mMMgCl2asneededasneededseenotes
I-5Enzyme0.5-1ul1-2ul 
Water(ddH2O)upto25µlupto50µl 
    
    
ThermocyclingConditions   
    
3StepPCR   
StepTemperatureTime 
InitialDenaturation98°C2minutes 
Denaturation98°C10seconds25-35cycles
Annealing55°C-68°C10-15seconds 
Extension72°C10-15seconds/kb 
FinalExtension72°C1-5minutes 
 4°CHold 
    
2StepPCR(seenotes)   
StepTemperatureTime 
InitialDenaturation98°C2minutes 
Denaturation98°C10-15seconds25-35cycles
CombinedAnnealing&Extension72°C15-30seconds/kb 
FinalExtension72°C1-5minutes 
 4°CHold 
    
Notes   
    
RecommendedDNATemplateaddition   
GenomicDNA50-250ng  
PlasmidDNA1pg-10ng  
ViralDNA1pg-10ng  
    
Mg2+   
ThefinalconcentrationofMg2+in1XI-5PCRMasterMixis1.5mM   
AddadditionalMg2+asneededin0.5mMincrements   
SuggestedfinalMg2+concentrationrangesfrom2mMto4mM   
    
2StepPCR   
Use2StepPCRisrecommendedwhentheprimerTmvaluesare>68°C   
    
PCRProduct/Cloning   
The2XI-5PCRMasterMixresultsinPCRproductwithbluntends   
Blunt-endcloningisrecommendedafterPCRwiththisproduct   
ForT/A-cloning,thePCRproductshouldbepurifiedbeforeA-addition