Description: Phi29DNAPolymeraseisresponsIBLeforthereplicationoftheBacillusSubtilisphagephi29(1).TheenzymeisahighlyprocessiveDNApolymerase(upto70,000baseinsertionsperbindingevent)withapowerfulstranddisplacementactivity(2)anda3'->5'proofreADIngexonucleasefunction(3).
SpecificActivity:100,000U/mg
Application: -Catalyzestheremovalof5'-mononucleotidesfromduplexDNA -Replicationrequiringahighdegreeofstranddisplacementand/orprocessivesynthesis -Highfidelityreplicationatmoderatetemperatures
Source: ArecombinantE.colistraincarryingthephi29DNAPolymerasegenefrombacteriophagephi29.
Suppliedin: 10mMTris-HCl 100mMKCl 0.1mMEDTA 1mMDTT 0.5%Tween-20 0.5%NP-40 50%Glycerol pH7.4@25°C
Suppliedwith: 10xphi29DNAPolymeraseReactionBuffer
10xphi29DNAPolymeraseBuffer 500mMTris-HCl 100mM(NH4)2SO4 40mMDTT 100mMMgCl2 pH7.5@25°C
UnitDefinition: 1unitisdefinedastheamountofpolymeraserequiredtoconvert0.5pmolofdTTPintoacidinsolublematerialin10minutesat30°C.
RecommendedStorageCondition:-20°C
References: 1.Blanco,L.andSalas,M.(1984)Proc.Natl.Acad.Sci.USA,81,5325-5329. 2.Blanco,L.etal.(1989)J.Biol.Chem.,264,8935-8940. 3.Garmendia,C.etal.(1992)J.Biol.Chem.,267,2594-2599. |