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商品详细MCLAB/Phi29 DNA Polymerase/PP-OEM/Any Size

MCLAB/Phi29 DNA Polymerase/PP-OEM/Any Size

商品编号： PP-OEM

品牌： Molecular Cloning Laboratories

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类： DNA聚合酶

公司分类： DNA_polymerase

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Phi29DNAPolymeraseisareplicativepolymerase.Thisenzymehas assistDNAreplication,phi29DNAPolymerasehasthehighestprocessivityandstranddisplacementactivityamongknownDNApolymerases-morethan70kblongDNAstretchescanbesynthesized.

Description:
Phi29DNAPolymeraseisresponsIBLeforthereplicationoftheBacillusSubtilisphagephi29⁽¹⁾.TheenzymeisahighlyprocessiveDNApolymerase(upto70,000baseinsertionsperbindingevent)withapowerfulstranddisplacementactivity⁽²⁾anda3'->5'proofreADIngexonucleasefunction⁽³⁾.

SpecificActivity:100,000U/mg

Application:
-Catalyzestheremovalof5'-mononucleotidesfromduplexDNA
-Replicationrequiringahighdegreeofstranddisplacementand/orprocessivesynthesis
-Highfidelityreplicationatmoderatetemperatures

Source:
ArecombinantE.colistraincarryingthephi29DNAPolymerasegenefrombacteriophagephi29.

Suppliedin:
10mMTris-HCl
100mMKCl
0.1mMEDTA
1mMDTT
0.5%Tween-20
0.5%NP-40
50%Glycerol
pH7.4@25°C

Suppliedwith:
10xphi29DNAPolymeraseReactionBuffer

10xphi29DNAPolymeraseBuffer
500mMTris-HCl
100mM(NH₄)₂SO₄
40mMDTT
100mMMgCl₂
pH7.5@25°C

UnitDefinition:
1unitisdefinedastheamountofpolymeraserequiredtoconvert0.5pmolofdTTPintoacidinsolublematerialin10minutesat30°C.

RecommendedStorageCondition:-20°C

References:
1.Blanco,L.andSalas,M.(1984)Proc.Natl.Acad.Sci.USA,81,5325-5329.
2.Blanco,L.etal.(1989)J.Biol.Chem.,264,8935-8940.
3.Garmendia,C.etal.(1992)J.Biol.Chem.,267,2594-2599.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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服务热线：4000-520-616

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手机：18915418616

官网：http://www.mclab.com/