Description: T7DNAPolymeraseisthemesophilic,highlyprocessive,andreplicativeDNApolymerasefrombacteriophageT7.Itisresponsiblefortherapidandaccuratereplicationofthevirusgenomeduringitsinfectioncycle.T7DNAPolymeraseisatwosubunitproteinthatconsistsofapolymerasedomain(gene5fromtheT7bacteriophage)andaprocessivityfactor(E.colitrxAgenethioredoxin)(1,2).Theenzymepossessesapowerful(3'->5')nucleaseactivitythatactsonbothsingleanddoublestrandedDNAandappearstoberesponsibleforthehighfidelityofthisenzymeandpreventsstranddisplacementsynthesis(3,4,5).
SpecificActivity:10,000U/mg
Application: Secondstrandsynthesisinsite-directedmutagenesisprotocols
Source: ArecombinantE.colistraincarryingthebacteriophageT7gene5.
UnitDefinition: 1unitisdefinedastheamountofpolymeraserequiredtoconvert10nmoloftotaldNTPsintoacidinsolublematerialin30minutesat37°C.
Suppliedin: 50mMKPO4 0.1mMEDTA 1.0mMDithiothreitol 50%Glycerol pH7.0@25°C
Suppliedwith: 10xT7DNAPolymeraseBuffer
10xT7DNAPolymeraseBuffer: 400mMTris-HCl 200mMMgCl2 500mMNaCl pH7.5@25°C
RecommendedStorageCondition:-20°C
Reference: 1.Grippo,P.etal.(1971)J.Biol.Chem.246,6867-6873 2.Modrich,P.etal.(1975)J.Biol.Chem.250,5515-5522 3.Adler,S.etal.(1979)J.Biol.Chem.254,11605-11614 4.Hori,K.,etal.(1979)J.Biol.Chem.254,11598-11604 5.Lechner,R.L.etal.(1983)J.Biol.Chem.258,11185-11196 |