MCLAB/SmartRT Reverse Transcriptase/SMRT-200/10000u(100 rxn)-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/SmartRT Reverse Transcriptase/SMRT-200/10000u(100 rxn)

MCLAB/SmartRT Reverse Transcriptase/SMRT-200/10000u(100 rxn)

商品编号： SMRT-200

品牌： Molecular Cloning Laboratories

市场价： ¥1780.00

美元价： 1068.00

产地：美国(厂家直采)

公司：

产品分类：反转录酶

公司分类： Reverse_transcriptase

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

SmartRT^TMReverseTranscriptaseisanengineeredMMLVRTthatimprovestheenzyme’sThermostABIlity,reducesRNaseHactivityanditsCDNAsynthesisability.Theenzymealsohasaterminaltransferaseactivity,whereitaddsafewextranucleotidestotheendofthesynthesizedcDNA. Witha3’modifiedoligodTprimeranda5’ SMARTuniversaloligocontainingaterminalcomplementationtonucleicacidsatthe3’ endofthefirst-strandcDNA,theSmartRT^TMreversetranscriptasewillproduceRACEreadyfull-lengthcDNA.

GeneralDescription

SmartRT^TMReverseTranscriptaseisanengineeredMMLVRTthatimprovestheenzyme’sthermostability,reducesRNaseHactivityanditscDNAsynthesisability.Theenzymealsohasaterminaltransferaseactivity,whereitaddsafewextranucleotidestotheendofthesynthesizedcDNA. Witha3’modifiedoligodTprimeranda5’ SMARTuniversaloligocontainingaterminalcomplementationtonucleicacidsatthe3’ endofthefirst-strandcDNA,theSmartRT^TMreversetranscriptasewillproduceRACEreadyfull-lengthcDNA.

Features
MCLAB'sreversetranscriptaseisoneoftranscriptasesthathasthehighestthermostabilitywithterminaltransferaseactivity.

Source
E.coli

Applications
ReversetranscriptionandRACEreadyfulllengthcDNAsynthesis

Suppliedwith
5xfirststrandcDNAsynthesisbuffer

RecommendedStorageCondition
-20°C

UnitDefinition
Oneunitisdefinedastheamountofenzymethatwillincorporate1nmolofdTTPintoacid-insolublematerialin10minutesat37°Cusingpoly(A):oligo(dT)astemplate:primer.

ShippingCondition
dryice

Userprotocol
ForRACEreadyfirst-strandcDNASynthesis
Combinethefollowinginseparate0.2-mlPCRtubes(20µlreaction):
1–9µlRNAsample
1µl5’SMARTuniversalPrimer
1µloligodTPrimer
1µldNTP
AddsterileH₂Otoafinalvolumeof12µlforeachreaction.
Incubatethetubesat65°Cfor5min
Coolthetubesonicefor3min
Addthefollowingtoeachreactiontube:
4µl5XFirst-StrandBuffer
1µlDTT(20mM)
2µldNTPMix(10mM)
2µlSmartRTreversetranscriptase
Incubatethetubes:
50°Cfor1.5hr
85°Cfor5min

Forregularfirst-strandcDNASynthesis

Combinethefollowinginseparate0.2-mlPCRtubes(20µlreaction):
1–10µlRNAsample
1µloligodTPrimerorRandomprimer
1µldNTP
AddsterileH₂Otoafinalvolumeof12µlforeachreaction.
Incubatethetubesat65°Cfor5min
Coolthetubesonicefor3min
Addthefollowingtoeachreactiontube:
4µl5XFirst-StrandBuffer
1µlDTT(20mM)
2µldNTPMix(10mM)
2µlSmartRTreversetranscriptase
Incubatethetubes:
50°Cfor45min
85°Cfor5min

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.mclab.com/