ProductDescription MCLAB's All-In-One5XReverseTranscriptionMixKitisoptimizedtosynthesizefirststrandcDNAforRT-PCRandRT-qPCR.MCLAB'sQuantumScript™HDReverseTranscriptaseisanengineeredmutationwithincreasedsensitivity,improvedspecificity,maximumThermostABIlityandreducedRNaseHactivity.Theoptimalfist-strandcDNAsynthesistemperatureforthisenzymeis50°C.ThehighertemperaturesreversetranscriptionreactionimprovescDNAyieldsfordifficultRNAtranscription.ThisPre-mixed5Xconcentratedsolutionincludesallnecessarycomponentsforfirst-strandsynthesis:5Xbuffer,dNTPs,MgCl2,DTT,primers(optimizedmixtureoligo(dT)andrandomprimers),RNaseinhibitors,QuantumScript™HDReverseTranscriptase,ItwillsimplifyyourfirststrandcDNAsynthesisprocess. Features Easy-to-usepre-mixedpackage Highfidelity,yieldandsensitivity Broaderlinearrange CompletecDNAsynthesisguaranteed Maximumsamplevolumeavailable Robustandhighthroughput Workwithdifficultsequences Applications RNAreversetranscription RecommendedStorageCondition -20°C ShippingCondition dryice |