Description: T7RNAPolymeraseisaDNA-dependentRNApolymerasederivedfromtheT7bacteriophagewhichexhibitsahighrecognitionspecificitytotheT7promoterandterminatorsequencesandcatalyzesthe5'->3'synthesisofRNAstartingataT7promotersequence(1,2).
SpecificActivity:312,500U/mg
Application: -RADIolabeledRNAprobepreparation -RNAgenerationforinvitrotranslation -RNAgenerationforstudiesofRNAstructure,processingandcatalysis -Expressioncontrolviaanti-senseRNA
Source: PurifiedfromastrainofE.colithatexpressestherecombinantT7RNAPolymerasegene.
Suppliedin: 50mMTris-HCl 100mMNaCl 1mMDTT 1mMEDTA 50%Glycerol 0.1%TritonX-100 pH7.9@25°C
Suppliedwith: 10xT7RNAPolymeraseBuffer 400mMTris-HCl 60mMMgCl2 100mMDTT 20mMSpermidine pH7.9@25°C
UnitDefinition: Oneunitisdefinedastheamountofenzymethatwillincorporate1nmolofATPintoacid-precipitablematerialin1hourat37°C.
RecommendedStorageCondition:-20°C
References: 1.Chamberlin,M,etal.(1973)J.Biol.Chem.248,2235-2244,2245-2250. 2.Chamberlin,M.etal.(1982)inTheEnzymes,3rdedition,ed.P.D.Boyer(AcademicPress,NewYork.)15,87-108. |