Introduction HumanTrypsin1,encodedbythePRSS1gene,isalsoknownascationictrypsinogen.Itcontainsasignalpeptide(residues1‑15),aproregion(residues16‑23),andamaturechain(residues24‑247).Trypsinispartoftheserineproteasefamily.TrypsincleaveslysineandarginineattheC-terminalsideofthepeptide.TrypsinoptimumpHispH-7to9.Theenzymeisinhibitedbyserineproteaseinhibitors,e.g.PMSF,andbymetalchelatingagents,e.g.EDTA. ProductDescription RecombinantHumanTrypsin-1isageneticallyengineeredproteinexpressedinE.coliandpurifiedbystandardchromatographytechniques.RecombinantHumanTrypsin-1isfreefromanyanimalandhumansources.TherearenocontaminatingenzymeactivitiessuchascarboxypeptidaseAandchymotrypsin.NoproteaseinhibitorssuchasPMSFarecontainedinthepreparation. Source:Humantrypsin-1,expressedinE.coli,withaC-terminal6-Histag. Purity:>95%asdeterminedbySDS-PAGE  | Lane: 1.MCLAB'sTrypsin 2.TrypsinfromsupplierS |
UnitDefinition:OneUSPunitoftrypsinactivitywillproduceaDeltaA253of0.003perminuteinareactionvolumeof3.0mlatpH7.6and25℃,withBAEEasasubstrate(1cmlightpath). Specificactivity:≥2500USPU/mg Storage:Storeat-20°C. Avoidmultiplefreeze-thawcycles. |