Description: SUMO(SmallUbiquitin-likeMOdifiers)ProteaseisahighlypurifiedUlp1fromaE.coliexpressionsystem,whichcarriestheyeastUlp1.ItrecognizesthetertiarystructureofSUMOandcleavesSUMOfromrecombinantfusionproteins.TheSUMOproteasehasaN-terminalHis-tagandcanberemovedbyNi-NTAagarose. Application: -Removaloffusiontagsfromrecombinantproteins -HighlydynamicandprecisecleavagecapABIlities -Purificationofproteinsandpeptides
Suppliedwith: 10Xhighsaltand10XnosaltSUMObuffers
10×SUMOProteaseBuffer(Saltplus) 500mMTris-HCl,pH8.0 2%Igepal(NP-40) 1.5MNaCl 10mMDTT
10×SUMOProteaseBuffer(Saltfree) 500mMTris-HCl,pH8.0 2%Igepal(NP-40) 10mMDTT SUMOProteaseStorageBuffer 25mMTris-HCl,pH8.0 0.1%Igepal(NP-40) 250mMNaCl 0.5mMDTT 50%glycerol Source:E.coli M.W.:26kDa Purity:≥95% Label:His-tag Activity:>105Unit/mg
QualityControl: SUMOProteasehasgreaterthan95%puritywithnonon-specificproteasecontamination.Itisfunctionallytestedfortheabsenceofanynon-specificproteaseactivity.
UnitDefinition: OneunitofSUMOProteaseisdefinedastheamountofenzymeneededtocleave85%of2µgofsubstrateproteinat30°Cinonehour.
RecommendedStorageCondition:-80°C Figures:
 Figure1,CoomassiestainingofSUMOProteaseindifferentelutionfractions.
 Figure2,RP-HPLCChromatogramofSUMOProtease. |