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商品详细MCLAB/TEV蛋白酶/TEP-100/1mg
MCLAB/TEV蛋白酶/TEP-100/1mg
MCLAB/TEV蛋白酶/TEP-100/1mg
商品编号: TEP-100
市场价: ¥3500.00
美元价: 2100.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白酶类
公司分类: protease
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

TEV(Tobaccoetchvirus)Proteaseisahighlysite-specificcysteineproteasethatrecognizesthecleavagesiteofGlu-Asn-Leu-Tyr-Phe-Gln-GlyandcleavesbetweenGlnandGly.TEVproteaseisaveryusefulreagentforcleavingfusionproteinsduetoitshighspecificityandhighactivityrate.

Description:
TEV(TobaccoEtchVirus)Proteaseisahighlysite-specificcysteineproteasethatrecognizesthecleavagesiteofGlu-Asn-Leu-Tyr-Phe-Gln-GlyandcleavesbetweenGlnandGly.TEVproteaseisaveryusefulenzymeforcleavingfusionproteinsduetoitshighspecificityanditshighactivityrate.TEVProteasecontainsanN-terminalHis-tag,anditcanberemovedbyNi-NTAcolumnaftercleavagereaction.

Application:
-Removaloffusiontagsfromrecombinantproteins
-HighlydynamicandprecisecleavagecapABIlities
-purificationofproteinsandpeptides

SuppliedWith:
TEVsubstrate,50µl
10XTEVbuffer

Source:
E.coliderivedfromTobaccoEtchVirus.

Label:His-tag

ReactionConditions:
TEVproteaseismaximallyactiveat34°C,butitsrecommendedtoperformdigestsatroomtemperature(20°C)or4°C.TheactivityofTEVproteaseisapproximately3-foldgreaterat20°Cthanat4°C.

10XTEVbuffer
500mMTris-HCl,1.5MNaCl,pH8.0

RecommendedStorageCondition:-20°C

Figures:
turbotev01.png
Figure1
turbotev02.png
Figure2,69.8kDaTEVsubstrateproteinisincubatedwithTurboTEV.Thecleavedproductsare41.2kDaand27.7kDa,respectively.

品牌介绍
MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.