MCLAB/Choo-Choo克隆试剂盒/CCK-384/1个-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/Choo-Choo克隆试剂盒/CCK-384/1个

MCLAB/Choo-Choo克隆试剂盒/CCK-384/1个

商品编号： CCK-384

品牌： Molecular Cloning Laboratories

市场价： ¥97920.00

美元价： 58752.00

产地：美国(厂家直采)

公司：

产品分类：克隆试剂盒

公司分类： Cloning_kit

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Choo-ChooCloning^TMKitsarehighlyefficientdirectionalPCRcloningkitsforrapid,ligaseandrestrictionenzyme-independentPCRcloning.

Description:
Choo-ChooCloning^TMKitsarehighlyefficientdirectionalPCRcloningkitsforrapid,ligaseandrestrictionenzyme-independentcloningofPCRproducts.TheyallowtocloneanyPCRfragmentintoanylinearizedvectoratanylocation.Bysimplyincubatingonice,theendsofaPCR-generatedDNAfragmentcanbepreciselyfusedintoaDNAvectorwith6bp(ormore)overlappinghomologoussequence.Thesystemisveryrobust.Upto8PCR-generatedDNAfragmentscanbeassembledandclonedintoonepiece(upto10kbinonestep).Thesystemishighlyefficient,with98-100%positivecolonies.

Function:
ThefunctionofChoo-ChooCloning^TMKitsdependonMCLAB'sproprietaryenzymesystems.Thereisnoneedforrestrictionenzymedigestion,ligation,orblunt-endpolishing.Anyextraunwantedbasescanbeeliminated inthefinalconstruct.ThelinearizedvectorcanbegeneratedbyPCRorrestrictionenzymedigestion.ThePCRfragmentscanbegeneratedbyTaqDNApolymeraseorotherhighfidelityDNApolymerases.TheadditionofaAbyTaqDNApolymeraseisnotrequiredandhasnoeffectoncloningefficiency.IfthePCRproductisamplifiedfromaplasmidtemplate,thenitneedstobegel-purifiedtoreducebackground.InadditiontoPCRcloning,Choo-ChooCloning^TMKitsalsohavethefollowingaplications:adaptor,linkerandtagadditionbeforeandaftertheinserts,andgenesynthesis.

Features:
-Cloneanyinsertintoanyvectoryouchooseatanylocation
-Norestrictiondigestion,phosphatasetreatment,orligationrequired
-Multiplefragments(upto8pieces)
-BroadPCRsizes(upto10kb)
-Finalconstructsareseamlesswithnoextraorunwantedbasepairs
-Simple45minutesingle-tubereactiononiceprotocol
-Highefficiencywith>=98%positivecolonies
-Multiplefunctions:compatIBLewithadaptor,linkerandtagadditionbeforeoraftertheinserts;compatiblewithgenesynthesis
-Highthroughputapplication

RecommendedStorageConditions:
StoreBox1at-20°C.
StoreBox2at-80°C.

Components:
TheChoo-ChooCloning^TMKitsareavailablein10-,20-,100-,and96-reactionsizes.

Cat#	Box1		Box2
	10xChoo-ChooCloning^TM ReactionBuffer	Choo-ChooCloning^TM EnzymeMix	DH5a Competent E.coli cells
CCK-10(10rxn)	20µl	20µl	50µlx10tubes
CCK-20(20rxn)	40µl	40µl	50µlx20tubes
CCK-100(100rxn)	200µl	200µl	50µlx100tubes
CCK-096(96rxn)	96X5µl		96x50μl(96-wellplate)
CCK-384(384rxn)	384X5µl		8x2.5ml

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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