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当前位置: 首页 > 产品中心 > Routine_cell_strain > MCLAB/Dh10 Beta/Dh10-100/1 Ea
商品详细MCLAB/Dh10 Beta/Dh10-100/1 Ea
MCLAB/Dh10 Beta/Dh10-100/1 Ea
MCLAB/Dh10 Beta/Dh10-100/1 Ea
商品编号: DH10-100
市场价: ¥2200.00
美元价: 1320.00
产地: 美国(厂家直采)
公司:
产品分类: 常规细胞株
公司分类: Routine_cell_strain
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
TheDH10BcompetentE.colihasatransformationefficiencyof1x109cfu/µgforsupercoiledDNAandisidealforhigh-efficiencycloningandplasmidpropagation.Itallowsstablereplicationofhigh-copynumberplasmids.IncomparisonwithDH5a,methylatedDNAfromgenomicpreparation couldbeefficientlytransformedintoDH10B,therefor,theDH10BcellsaremoreapplicablethanDH5aingeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresidues.

Description:
TheDH10BcompetentE.colihasatransformationefficiencyof1x109cfu/µgforsupercoiledDNAandisidealforhigh-efficiencycloningandplasmidpropagation.Itenablesstablereplicationsofhigh-copynumberplasmids.IncomparisonwithDH5a,methylatedDNAfromgenomicpreparation couldbeefficientlytransformedintoDH10B,thereforDH10BcellsaremoreapplicablethanDH5aingeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresidues.

Application:

  • Highesttransformationefficiency
  • Generalcloning
  • Blue-whiteselection
  • ReducedrecombinationofclonedDNA(recA1)

Recommendedstoragecondition:
Thisproductshouldbestoredat-80°C.Thawoniceonlybeforeuse.Donotre-freeze.

Genotype:
F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1deoRΔ(ara,leu)7697araD139galUgalKnupGrpsLλ--

 CompetentCellType:
Chemically

TransformationEfficiency(cfu/μg):
>1x109

Blue-WhietScreening:
Yes

Strain:
K12

ReducesRecombination:
Yes

CloningMethylatedDNA:
Yes

ImprovesPlasmidQuality:
Yes

PreparingUnmethylatedDNA:
Notsuitable

T1PhageResistant:
No

RecADeficien:
Yes

品牌介绍
MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.