Description: TheDH10BcompetentE.colihasatransformationefficiencyof1x109cfu/µgforsupercoiledDNAandisidealforhigh-efficiencycloningandplasmidpropagation.Itenablesstablereplicationsofhigh-copynumberplasmids.IncomparisonwithDH5a,methylatedDNAfromgenomicpreparation couldbeefficientlytransformedintoDH10B,thereforDH10BcellsaremoreapplicablethanDH5aingeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresidues. Application: - Highesttransformationefficiency
- Generalcloning
- Blue-whiteselection
- ReducedrecombinationofclonedDNA(recA1)
Recommendedstoragecondition: Thisproductshouldbestoredat-80°C.Thawoniceonlybeforeuse.Donotre-freeze. Genotype: F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1deoRΔ(ara,leu)7697araD139galUgalKnupGrpsLλ-- CompetentCellType: Chemically TransformationEfficiency(cfu/μg): >1x109 Blue-WhietScreening: Yes Strain: K12 ReducesRecombination: Yes CloningMethylatedDNA: Yes ImprovesPlasmidQuality: Yes PreparingUnmethylatedDNA: Notsuitable T1PhageResistant: No RecADeficien: Yes |