MCLAB/2X HotSybr qPCR套件/HSM400/1个-免疫在线蚂蚁淘旗下平台

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MCLAB/2X HotSybr qPCR套件/HSM400/1个

商品编号： HSM400

品牌： Molecular Cloning Laboratories

市场价： ¥2980.00

美元价： 1788.00

产地：美国(厂家直采)

公司：

产品分类：定量PCR

公司分类： real_time_PCR

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Sybrgreendyebased4x1.25mlfor200reactions.Designedforallkindsofreal-timePCRmachines.

RoxReferenceDye	Instrument	CatalogNo.
NoRox	BioRadiCyclerMiniOpticon,Opticon2,Chromo4,iQ5;RocheLightCycler480;MJResearchDNAEngineOpticon2,Chromo4;CorbettRotogene3000,6000	HSM400RF
LowRox	ABI^®7500qPCRSystems,ViiA™7,QuantStudio™12KFlex,AgilentMx3000P™Mx3005P™andMx4000™	HSM400LR
RegularRox	ABI^®PRISM^®7000,7700,7900HT,ABI^®7300qPCRSystems,GeneAmp^®5700,StepOne™,andtheStepOnePlus™	HSM400

ForResearchUseOnly.

Figure:AmplificationofhumanGAPDHgenetargetwith2XHotSybrReal-timePCRKit.Amplificationcurvesareshownfortenfolddilutionsof0.0002pMto20pMofplasmid.Insetsshowthedissociationprofileandthestandardcurvedata.

Description:
Thisisahighperformancereal-timePCRreagent.ItutilizesMCLAB'sproprietaryquantitativePCRtechnology.

Advantages:
2xHotSybrPCRReactionMixproductscutthetotalreactiontimedowntohalf.

-Normallythetotalreactiontimeis4350seconds:95°C,10minutes=>(95°C,15seconds=>60°C,60seconds)x50
-ForMCLAB's2xHotSybrPCRReactionMix,thetotalreactiontimeisreducedto2350seconds:95°C,10minutes=>(95°C,5seconds=>60°C,30seconds)x50

Application:
SybrgreendyebasedquantitativePCR:includingDNAquantification,2-stepRTPCR,SNPanalysis,etc.

Primerandprobedesign:
Appropriatesoftware,suchasABIPrimerExpress^TM,shouldbeused.

RecommendedReactionConditions:
95°C,10minutes->(95°C,5seconds.->60°C,30seconds.)for50cycles->meltingcurve.

RecommendedStorageConditions:-20°C

Notes:
Toachieveaccuratequantification,itishighlyrecommendedto(1)doreplicates;(2)reducepipettingerror;(3)primerconcentrationfrom100nMto300nM;(4)runmeltingcurvefollowingamplificationcycles.

Reference:
HiguchiR,DollingerG,WalshPS,GriffithR;Bio/Technology10:413-417,1992

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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