Description:
Small,non-codingmiRNAsarewidelypresentineukaryotes.ManystudiesevidencedthatmiRNAscontrolmanyimportantphysiologicalprocessesincelldevelopmentanddifferentiation.Therefore,quantitativeassayingofmiRNAisimportantinbothbasicandappliedresearch.
miRNAcDNASynthesisKitprovidesauniversalfirst-strandcDNAsynthesissystemwhichcombinesoptimizedpolyadenylationwithreversetranscriptionreactionsbasedontheproprietaryhighqualityenzymes.TherobustkitisidealformostreliablefirststrandsynthesiswithhighercDNAyields,sensitivity,andaccuratequantitationfrom10pgto1µgoftotalRNAinput.
Theuniversalfirst-strandcDNAsynthesisallowsthedetectionofmRNAspecies,includingβ-ActinandGapDH,fromthesamecDNAsample.ThemiRNA-specificamplificationcouldbedonewithtarget-specificPCRforwardprimerduringthesubsequentPCRreaction.WhencomparedtotrADItionalhybridization-baseddetectionmethods,suchasNorthernblotanalysis,theqRT-PCRmethodisfaster,morespecific,moresensitiveandusinglesssamplematerial.

Features:
-EfficientreversetranscriptionofmiRNAsintocDNAinasinglestep.
-PrecisequantitativeandaccuratemeasurementofmiRNAexpressionprofileswithUniversalqPCRPrimerincludedinthekitgivesyoutheflexibilitytoorderyourqPCRdetectionreagentsseparately.
-DifferentiationbetweenmatureandprecursormiRNA.
-ProfilingofsmallRNAs,miRNAs,ormRNAsfromasinglecDNAsynthesisreaction.

RecommendedStorageCondition:-20°C

References:
1.Drummond,D.R.etal.(1985)J.Cell.Biol.100,1148.
2.Galili,G.etal.(1988)J.Biol.Chem.263,5764.
3.Lingner,J.andKeller,W.(1993)NucleicAcidsRes.21,2917.