Description: DirectDNASolution isforpurifyinggenomicDNAfromavarietyofsampletypes,suchaswholeblood,tissues,bonemarrow,buffycoat,bodyfluids,culturedcells,cellsuspensions,andGram-negativebacteria.ThismethodemploysauniquepurificationmatrixandreagentsforlysingcellsandcapturingDNA.Itiswell-suitedforstandardPCRscreeningapplications.Thereisnoneedtopre-lysethesampleorprecipitatetheDNA,andthepurifiedDNAisreadytouseinstandardPCR. DirectDNASolutionProtocol 1.TakeoutthewholesampleorpartofitforDNAextraction.Washitifnecessary. 2.Mechanicallypulverizethesampleintoamush.Forexample,sliceorgrindthesample. 3.Transferpulverizedsampletoasteriletubeandmixwellwith100uL(orothervolumethatcanimmersethesample)ofDirectDNASolution. 4.Incubateovernightat37Cinanincubator. 5.Spinthetubeatmaximumspeedfor10min. 6.Use1uLofthesupernatantastemplateinPCRreaction(25uL)withcompatIBLePCRmastermix,forexample,2XBlueStar™PCRMasterMix. |