Description: Oligod(T)18-24 MagneticBeadsarepolymerbasedaffinitymatrixforthesmall-scale,rapid isolationofmRNAfromcrudecelllysatesandtissues.Theisolationoccursthroughthehybridizationofcovalentlycoupledoligod(T)18-24 tothepoly(A)regionpresentinmosteukaryoticmRNA.TheisolatedmRNAcanbeuseddirectlyinmostdownstreamapplicationsinmolecularBIOLOGy,e.g.RT-PCR,CDNAlibraryconstruction,invitrotranslationexperiments,andgeneexpressionanalysis. Theprotocolcanbeperformedin15minutes,withouttheneedtopreparetotalRNAorperformanyotherpurificationsteps.TheoligodTboundtothebeadsurfacecanbeusedtobothcapturethemRNAandactasaprimerforreversetranscriptaseduringfirststrandcDNAsynthesis.Oligod(T)18-24MagneticBeadsarealsoavailablein96-wellplateformatforhighthroughputusage. Oligod(T)18-24 MagneticBeadsaresuppliedasa5mg/mlsUSPensioninphosphatebufferedsaline(PBSbuffer,pH7.4),containing0.05%Tween® 20and0.05%NaN3 . Applications: RNAExpressionAnalysis RNADetection&Isolation StorageTemperature 4°C StorageBuffer 250mMTris-HCl,pH7.5 20mMEDTA 0.1%Tween®20 0.02%NaN3 |