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manuals_protocolsmanuals_protocols    
ProtocolforhumanProtocolformouse    

Table:Validatedprimersetsforhumanandmousehousekeepinggenesandtheirexpressionlevels.


HumanGenes
MouseGenes
Name
GeNEBankAccession#
RelativeExpression*
Name
GeneBankAccession#
RelativeExpression*
h18SrRNA
X0325
****
m18SrRNA
K01364
****
hActß
NM_001101
***
mAct²
NM_007393
***
hB2m
NM_004048
***
mB2m
NM_009735
***
hGapdh
NM_002046
***
mGapdh
NM_008084
***
hPpia
NM_021130
***
mPpia
NM_008907
***
hRpl13a
NM_012423
***
mRpl13a
NM_009438
***
hRplp0
NM_001002
**
mHsp90ab1
NM_008302
**
hHprt1
NM_000194
**
mLdhal6b
NM_175349
**
hTfrc
NM_003234
**
mHprt1
NM_013556
**
hHsp90ab1
NM_007355
*
mGusb
NM_010368
*
hGusb
NM_000181
*
mTbp
NM_013684
*
hUbc
NM_021009
*
mTfrc
NM_011638
*

*Summaryoftheexpressionlevelsofgenesincomparisonwith18SrRNAusingoursensitivityhumanormousereferenceCDNAandourproprietarySYBRgreenmastermix.

RelatedProducts:qHRcDNA,HSM,HTP

ReactionConditions:
95°C,10minutes->(95°C,15seconds.->60°C,30seconds.)for40cycles->meltingcurve.

RecommendedStorageCondition:-20°C


Figure:Standardcurveforhousekeepinggenehprt1expressionatmediumlevels.

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MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.