
MCLAB’sTopomizeAmpliconLibraryPrepKitisaninnovative,fastandhighqualityAmpliconlibraryconstructionkitfornextgenerationsequencing.
Description: MCLAB’sTopomizeAmpliconLibraryPrepKitisaninnovative,fastandhighqualityAmpliconlibraryconstructionkitfornextgenerationsequencing.Targetedsequencingoflargecohortsofsamplesisapowerfultoolforthediscoveryanddetectionofdisease-causingvariantsassociatedwithmanyinheriteddiseasesandcancers.AfterPCRamplificationofthetargetedDNAregionstogenerateampliconpools,MCLAB’sTopomizeAmpliconLibraryPrepKitcanbeusedtoquicklyaddadapterswithbarcodesfordeepsequencing. InsteadofusingtrADItionalligase-basedmethods,ourkitusestopoisomerase-basedtechnologytoattachadapterswithbarcodesontotheamplicons.TheTopomizeAmpliconLibraryKithasunparalleledefficiencyandyieldwithnoadapterdimersorchimeras.Thekitisdesignedfor10ngto1µgofeachpooledampliconDNAinput,andiscompatIBLewiththeIlluminaplatforms.Thefastworkflowallowstheampliconlibrarytobereadyinlessthan40minutes. Highlights:
Figure: TopomizeAmpliconLibraryPrepWorkflow |