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商品详细MCLAB/MCNext&trade；DNA样本制备试剂盒/MNEXT-24/1 Ea

MCLAB/MCNext&trade；DNA样本制备试剂盒/MNEXT-24/1 Ea

商品编号： MNEXT-24

品牌： Molecular Cloning Laboratories

市场价： ¥19800.00

美元价： 11880.00

产地：美国(厂家直采)

公司：

产品分类： DNA文库制备试剂盒

公司分类： DNA_library_preparation

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

TheMCNextDNASamplePrepKitisahighperformance,cost-effectivesampleprepsolutionforNGSusingtheIlluminaplatforms.

Description

KitFeatures

TheMCNextDNASamplePrepKitisahighperformance,cost-effectivesampleprepsolutionforNGSusingtheIlluminaplatforms.Abead-based,gel-freesizeselectionworkflowinonly90mineliminatestheneedformechanicalfragmentationandagarosegelsinsizeselection,theavailABIlityofpair-ends96indicesfacilitatesmultiplexhigh-throughputapplications.TheMCNextDNASamplePrepKitincorporatesdifferentflankingsequenceadditionsbyDialatum^TMtransposome(USpatentpending),resultinginfewerprimer-dimersandabettersignal/noiseratioinsequencingoutcomes.

FastPrepWorkflow

Completetheprotocolin90minutes
Enzymaticfragmentationwithoutsonication
One-stepfragmentation/taggingtosavetime

LowInputandHighThroughput

Maximumof50nginputDNAneeded
Pre-mixedenzymesandnucleotidesformultiplexedprocessing
Plate-basedprocessingcompatIBLeforupto96samplesperkit

HigherCoverageRateandFullCompatibility

Directionaltransposomedoublesthesequencingcoverage
WorksonallIlluminaNGSplatforms

BestValueonMarket

All96Illuminaindicesincluded

MCNext DNA Sample Prep Kit figure 1.jpg

Figure1.MCNext^TMSamplePreparationKitworkflowtakes90mintofinishthesampleprep.

MCNext DNA Sample Prep Kit figure 2.jpg

Figure2.MCNext^TMSamplePreparationKitfragmentsanddirectionallytagsDNAinasinglesteptoboostsequencingcoverageandsignalstrength.Subsequentlimited-cyclePCRamplificationaddsdualindicestoenableupto96librariestobepooledandsequenced.

KitCompatibility

IlluminaGenomeAnalyzerIIx
Illumina HiScanSQ
Illumina HiSeq1000
Illumina HiSeq2000
Illumina HiSeq2500
Illumina MiSeqDesktopSequencerv1,v2andv3
Illumina NextSeq500Sequencer

Questions&Answers

Q:WhatarethemainprotocoldifferencesbetweentheMCNextDNASamplePrepKitandtheconventionalNGSDNASamplePrepkit?
A:TheMCNextDNASamplePrepkitutilizesasingle-wellenzymaticreactionforbothfragmentationandadaptersadditionforlibraryconstruction,sothereisnomechanicalfragmentation/shearingrequiredforDNAsampleprep.Thewholestreamlinedsampleprepprocessonlytakes90mintocompletecomparingtoawholedayworkflowof theconventionalNGSpreps.
Q:WhatarethesimilaritiesbetweentheMCNextDNASamplePrepKitandtheIlluminaNexteraDNASamplePrepKit?
A:Bothkitsutilizeenzymaticfragmentationwithoutsonicationandspeeduplibraryconstructionbyonestepfragmentation/tagging;bothkitsrequirelowDNAinputwith50ngrange;bothkitsareoptimizedformultiplexingupto96samplesandbothkitstakeasshortas90mintocompletethesampleprep.
Q:WhataretheadvantagesoftheMCNextDNASamplePrepKitcomparingwithIlluminaNexteraDNASamplePrepKit?
A:MCNextDNASamplePrepKitprovidestheDialatum^TMtransposome(USpatentpending)inthetagmentationstepwithdifferentflankingsequencesaddedpriortotheP5-Read1/Read2-P7addition.Thisflankingsequencedifferentiationsignificantlyenhancesthelibraryconstructionefficiency,meanwhile,minimizesprimer-dimerformationcomparingwiththeunilateraladapteradditionbyIlluminaNexteraDNASamplePrepKit,whichallowsmorePCRcyclesinthesubsequentamplificationstepforabettersignal/noiseratio. TheotheradvantageisthattheMCNextDNASamplePrepKitcontainsallsequencingindicesforIlluminaplatforms,anextrasavingforcustomers.
Q:WhataretheadditionalcomponentsIneedtoordertocompletetheprep?
A: SincetheIlluminasequencingindicesareallincludedinthiskit,customersonlyneedtoordertheZymo™PurificationKit,theBeckmanCoulterAgencourtAMPureXP60mlKit, andsomegenerallabconsumablesdescribedintheusermanual.
Q:ArekitcomponentssuchasprimermixandbuffersinterchangeablebetweenMCNextDNASamplePrepKitandIlluminaNexteraDNASamplePrepKit?
A: No.ThereagentsarenotinterchangeablebecauseinthetagmentationsteptheDialatum^TMtransposomehasadifferentoligoinsertionandtheIlluminatransposomeproducesaunilateraladapteraddition,thelibraryproductsdifferfromtheverybeginningofthesampleprep,eventheworkflowsaresimilar,butthechemistryisdifferentdownstream.

Illumina®isaregisteredtrademarkofIllumina,Inc.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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手机：18915418616

官网：http://www.mclab.com/