MCLAB/2X MCAmp Library Amplification Master Mix + 10X Primer Set/LIBAP-250/1 Ea-免疫在线蚂蚁淘旗下平台

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商品详细MCLAB/2X MCAmp Library Amplification Master Mix + 10X Primer Set/LIBAP-250/1 Ea

MCLAB/2X MCAmp Library Amplification Master Mix + 10X Primer Set/LIBAP-250/1 Ea

商品编号： LIBAP-250

品牌： Molecular Cloning Laboratories

市场价： ¥6200.00

美元价： 3720.00

产地：美国(厂家直采)

公司：

产品分类：其他

公司分类： Other_molecular_reagents

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

The2XMCAmplibraryamplificationmastermixhasbeenspeciallyformulatedtoprovideaccurateandrobustamplificationofNGS(next-generationsequencing)libraryfragments.

Description
The2XMCAmplibraryamplificationmastermixhasbeenspeciallyformulatedtoprovideaccurateandrobustamplificationofNGS(next-generationsequencing)libraryfragments.Themastermixutilizesaproprietarycombinationofourultra-fast,high-fidelityDNApolymerases.Thepolymerasesallpossess3'->5'exonuclease(proofreADIng)activitywithaprocessivitytentimesgreaterthangenericDNApolymerases.ThereactionbufferhasbeenoptimizedtoproviderobustamplificationoflibraryfragmentsevenwithlowinputandGCorATrichsequences.Theresultingamplificationshaverobustyieldwithhigh-fidelity,lowPCRduplication,andlowbiaswhichproducesgreaterandmoreuniformcoverageespeciallyinchallenginggenomicregions.
ForIllumina^®platforms,anoptimized10XPrimerSettargetingtheP5andP7sitesisalsoavailable.

Producthighlights

High-fidelity: ThemastermixutilizesDNApolymerasesthatallpossess3'->5'exonuclease(proof-reading)activityforaccuratenucleotideincorporationsintoyourfinalproducts.
High-efficiency: Thepolymerasesinthemastermixhaveultra-highprocessivitysothatlesscyclesarerequiredforsequencereadylibraries.
LowbiasandPCRduplication: OptimizedbufferformulationtominimizeamplificationbiasandPCRduplication.Thisresultsinagreaternumberofreadswithmoreuniformcoverage,helpingtominimizecostandtime.

Recommendedstoragecondition:-20°C
HeatInactivation: No

UnitAssayConditions:
25mMTAPS-HCl(pH9.3@25°C),50mMKCl,2mMMgCl₂,1mMβ-mercaptoethanol,200μMdNTPsincluding[3H]-dTTPand400μg/mlactivatedCalfThymusDNA.

UnitDefinition
Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacidinsolublematerialin30minutesat74°C.

2X MCAmp library amplification master mix
Figure:LibrarypreparationandamplificationresultsfromvaryinginputquantitiesofshearedhumangenomicDNA.Librarypreparationandsizeselection(~350bp)wascarriedoutusingMCLAB'sTopomizeDNALibraryPrepKit.Allamplificationswerecarriedouttoeightcycles.
A.Agarosegelimageafterlibraryamplificationandcleanupshowingstablesizedistribution.M:100bpMarker.Lane1:100nginputDNA.Lane2:50nginputDNA.Lane3:25nginputDNA.B.AverageDNAyieldofcorrespondinggelagarosesamplesdeterminebyqPCRusingMCNexttmSYBR^®FastqPCRLibraryQuantifiactionKit.

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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