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商品详细MCLAB/RCA DNA Amplification Kit/PPK-OEM/1 Ea

MCLAB/RCA DNA Amplification Kit/PPK-OEM/1 Ea

商品编号： PPK-OEM

品牌： Molecular Cloning Laboratories

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：靶向测序试剂盒

公司分类： Targeted_sequencing_kit

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

RCADNAamplificationkitsarenovelproductsdevelopedspecificallytopreparetemplatesforDNAsequencing.TheRCAmethodutilizesbacteriophagephi29DNApolymerasetoexponentiallyamplifysingle-ordouble-strandedcircularDNAtemplatesbyrollingcircleamplification(RCA).ThisisothermalamplificationmethodproducesmicrogramquantitiesofDNAfrompicogramamountsofstartingmaterialinafewhours.

Description:
RCADNAamplificationkitsarenovelproductsdevelopedspecificallytopreparetemplatesforDNAsequencing.AsillustratedinFigure1,theRCAmethodutilizesbacteriophagephi29DNApolymerasetoexponentiallyamplifysingle-ordouble-strandedcircularDNAtemplatesbyrollingcircleamplification(RCA)^(1,2).ThisisothermalamplificationmethodproducesmicrogramquantitiesofDNAfrompicogramamountsofstartingmaterialinafewhours.

AmplificationinvitroofverysmallamountsoftemplateDNAeliminatestheneedforovernightcellcultureandconventionalplasmidorM13DNApurification.TheproofreADIngactivityofphi29DNApolymeraseensureshighfidelityDNAreplication.⁽³⁾

Thestartingmaterialforamplificationcanbeasmallamountofbacterialcellscontainingaplasmid,anisolatedplasmid,intactM13phage,oranycircularDNAsample.BacterialcoloniescanbepickedfromagarplatesandaddeddirectlytotheRCAreaction.Alternatively,microliterquantitiesofasaturatedbacterialcultureoraglycerolstockcanserveasstartingmaterial.Dependingonthesourceofstartingmaterial,amplificationiscompletedin4–18hoursat30˚Cwithnoneedforthermalcycling.TheproductoftheRCAreactionishighmolecularweight,double-strandedconcatemersofthecirculartemplate.

NotethatwhenstartingwithM13clones,theRCAproductisdouble-strandedDNAandcanbesequencedwithforwardandreverseprimers.DNAamplifiedbytheRCAmethodcanbeuseddirectlyincyclesequencingreactionswithoutanypurification.

Figure:
MCRCA DNA Amplification Kit figure 1.png
Figure1,SchematicdiagramoftheRCAprocess.RandomhexamerprimersannealtothecirculartemplateDNAatmultiplesites.Phi29DNApolymeraseextendseachoftheseprimers.WhentheDNApolymerasereachesadownstreamextendedprimer,stranddisplacementsynthesisoccurs.Thedisplacedstrandisrenderedsingle-strandedandavailabletobeprimedbymorehexamerprimer.Theprocesscontinues,resultinginexponential,isothermalamplification.

MCRCA DNA Amplification Kit figure 2.png
Figure2.

Components:

RCADNAAmplificationKit(100reactions)
Cat#:PPK-100
Phi29DNAPolymerase,20µl,(10,000units/ml)
2xMCRCAmix,1ml
CellLysisBuffer,1ml

RCADNAAmplificationKit(500reactions)
Cat#:PPK-200
Phi29DNAPolymerase,100µl,(10,000units/ml)
2xMCRCAmix,5ml
CellLysisBuffer,5ml

References:
1.Dean,F.etal.,GenomeResearch11,1095–1099(2001).
2.Lizardi,P.etal.,Nat.Genet.19,225–232(1998).
3.Estaban,J.A.etal.,J.Biol.Chem.268,2719–2726(1993).

品牌介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

公司介绍

MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB

品牌分类

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