Description: TheGel/PCRDNAIsolationSystemhasbeendevelopedtoisolateDNAfromagarosegel,PCRproducts,andotherenzymaticreactions.Withjustafeweasybindingandwashingsteps,Gel/PCRDNAIsolationSystemcanrecoverready-to-useDNAfromvarioussamplesinabout10-20minutes. Application: Gel/PCRDNAIsolationSystemprovidesreproducIBLeyieldsofhigh-purityDNAsuitableforuseinmostapplications,including: -AutomatedfluorescentandrADIoactivesequencing&PCR -Restrictiondigestion&modifyingenzymaticreaction -Ligation -labeling,&hybridization Features: -EfficientextractionofDNAfragmentsfrom100-bpto10-kbp -HighpurityofDNA(A260/A2801.8-1.9) ->95%primerandsaltsremoval -RecoverDNAfragmentsfromstandardoflow-meltingpointagarosegelsinTAEorTBEbuffer -EluteDNAwithjust15-30µlElutionbufferorddH2O -Recoveryupto90% -PreparationTime:10-15minutes -NosodiumIodidetointerferewithsubsequentreactions -NoshearingoflargeDNAfragments Suitablesample: -Agarosegel -PCRproduct -Enzymaticreactionwithnosodiumiodidetointerferewithsubsequentreactions DNAisolationsize:100bp-10kbp |