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当前位置: 首页 > 产品中心 > Other_molecular_reagents > MCLAB/BDX64缓冲区/BDX-100/1个
商品详细MCLAB/BDX64缓冲区/BDX-100/1个
MCLAB/BDX64缓冲区/BDX-100/1个
MCLAB/BDX64缓冲区/BDX-100/1个
商品编号: BDX-100
市场价: ¥5260.00
美元价: 3156.00
产地: 美国(厂家直采)
公司:
产品分类: 其他
公司分类: Other_molecular_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

MCLAB'sBDX64isaBigDye®enhancingbuffer,itisusedforDNAsequencing.BDX64bufferhasthesameionstrengthastheBigDye®premix3.1and1.1.

Description:
MCLAB'sBDX64isaBigDye®enhancingbuffer,itisusedforDNAsequencing.BDX64bufferhasthesameionstrengthastheBigDye®premix3.1and1.1.
Upto64(0.12µlbigdyein10µlreaction)ormorefolddilutionsbycombiningwiththe5xdilutionbuffer
Enhancesthepolymeraseactivityandreducestheextensiontimefrom4to1minute.
Reducesthesignaldeclinerateandresultsinevenpeakdistributions.
OptimizesforusingwithBigDye®Chemistry(ABI)on310,3100,3130/3130xl&3730/3730xl.
 

Application:

Component

Volume

Volume

  BigDye®3.1

0.25µl

0.5µl

  5xbuffer(cat#SBUF-100)

0.875µl

0.75µl

  BDX64buffer

2µl

2µl

  Template

100ng

100ng

  Primer

3.2pmol

3.2pmol

  AddH2Oto10µl

  

 

SequencingExamples:
1.Dilutionfold32X:


2.Dilutionfold64X:


3.Dilutionfold128X:


4.Dilutionfold256X:


SignalDistributions:beforeandafterBDX64wasadded


RecommendedCycleConditions:
96°C,3minutes.+30x(96°C,10seconds.50°C,5seconds.60°C,2minutes.)

RecommendedStorageCondition:-20°C

品牌介绍
MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.