Description:
TheBrightDye®TerminatorCycleSequencingKitisdesignedfordenovosequencing,resequencing, PCRproduct,plasmid,fosmid,andBACtemplatesbyutilizinghighlyflexiblechemistry.ThiskitusesfourdifferentfluorescentdyestolabelddNTPs,whichareaddedsequentiallytotheprimerthroughacyclesequencingreaction.Allrequiredreagentsforthesequencingprocedureareinareaction-ready,pre-mixedformat.BrightDyeterminatorkitiscompatiblewithcommerciallyavailableBigDyeterminatorV3.1kit,withoutanychangesintheworkflow.
Withenhancedrobustnessandbetterdyemobilitycharacteristics,theBrightDye®TerminatorCycleSequencingKitcanbeusedinawiderangeofapplicationswiththefollowingfeatures: - Longreadlengths.
- BetterperformancereADIngthroughGCrichregions.
- Longerreadswithmoreuniformpeakheights.
- Improvedproductivityforlesscost.
Applications:
Denovosequencing
Resequencing
Sequencingdifficulttemplates Long-readsequencing
Sequencingacrossalltemplatetypes(plasmids,PCRproducts,BACs,andfosmids)
Mixed-basedetection
SequencingshortPCRproductsusingrapidelectrophoresisrunmodules Platforms:
GeneAmp®9700,Veriti®ThermalCycler,3730DNAAnalyzer,3500xLGeneticAnalyzer,3500GeneticAnalyzer,310GeneticAnalyzer,3130GeneticAnalyzer,3130xlGeneticAnalyzer,3730xlDNAAnalyzer TemplateCompatibility:
PlasmidDNA(≤15Kb)
Fosmids
SingleStrandedDNA
LamBDaDNA
BACDNA
GenomicDNA(Bacterial)
PCRAmplicons Storage:
Storekitat-20°C.Avoidrepeatedfreeze-thawcycles. RegulatoryStatement:
ForResearchUseOnly.Notforuseindiagnosticprocedures. |
