IfyouareusingMCLAB'sDNAsequenceringservices,youdonotneedtopurchaseorsubmitanyprimerslistedhereinyourorders.YouonlyneedtospecifytheprimernamesinyourorderformandMCLABwilltakecareofeverythingrelatedtotheseprimers. WeprovidecustomoligonucleotidesynthesisserviceincombinationofourDNAsequencingservicetobetterassistourcustomers.Firsttimeprimersynthesisorderwillbefulfilledwithin24hours.Oncetheprimersaresynthesized,wealiquotthemfortheDNAsequencingandsavetherestintheMCEasyDepository™forpossIBLefutureusagefromourcustomers.Sothatthenexttimewhenyouorderthesequencingusingthesameprimers,youwillsavetimeandmoneybychoosingtheprimerswesavedforyouintheMCEasyDepository ™.Thisisacompletefreeservicefromustosaythanktoourcustomers.
WealsoprovideourcustomersfreetemplatesandprimersstorageinMCLAB'sfreezerarchivesforupto12monthsfromthetimeofsubmission.Thiswaywhenyouorderare-sequencingorsubmitothertemplatestobesequencedbythesamegroupofprimers,whatyouneedtodoisjusttelluswhichprimercombinationtouse.It'sjustlikethateasy. CustomerscouldalsogettheirDNAtemplatesand/orprimersbackbysimplyincludesuchinstructionsintheonlineorderformwhensubmittingtheirsequencingrequeststoMCLAB.It'slikethatsimple.
# | Primer | Sequences(5'd~3') | Plasmid | Manufacturer | 1 | 28glll | GTATGGGATTTTGCTAAACAAC | Ph.D.glll | NewEnglandBiolabs | 1a | -96glll | CCCTCATAGTTAGCGTAACG | Ph.D.glll | NewEnglandBiolabs | 2 | Ac5Forward | ACACAAAGCCGCTCCATCAG | pAc5.1/V5-His | Invitrogen | 3 | ADReverse | AGATGGTGCACGATGCACAG | AD | | 4 | a-Factor | TACTATTGCCAGCATTGCTGC | Pichia(pMET) | Invitrogen | 5 | AOX1Forward | GACTGGTTCCAATTGACAAGC | Pichia | Invitrogen | 6 | AOX1Reverse | GCAAATGGCATTCTGACATCC | Pichia | Invitrogen | 7 | ASHSVTagPrimer | ATCCTCGGGGTCTTCCG | pIEx | Novagen | 8 | ASSoTag18merPrimer | GTCCATGTGCTGGCGTTC | pIEx | Novagen | 9 | AUG1Forward | CAATTTACATCTTTATTTATTAACG | Pichia | Invitrogen | 10 | AUG1Reverse | GAAGAGAAAAACATTAGTTGGC | Pichia | Invitrogen | 11 | BacForward | TTTTACTGTTTTCGTAACAGTTTT | pBlueBac4.5 | Invitrogen | 12 | BacReverse | CGGATTTCCTTGAAGAGAGTA | pBlueBacHis2 | Invitrogen | 13 | Baculovirus(+15)Reverse | ACTTCAAGGAGAATTTCC | pMelBac | Invitrogen | 14 | BGHReverse | TAGAAGGCACAGTCGAGG | Universal | | 15 | BKReverse | ACAGGAAACAGCTATGACCTTG | BKvirus | | 16 | BKRSV | CGCCATTTGACCATTCA | pBK-rsv | | 17 | BluescriptKS | TCGAGGTCGACGGTATC | pBluescript | Stratagene | 18 | BluescriptSK | CGCTCTAGAACTAGTGGATC | pBluescript | Stratagene | 19 | CBDcenA | TCAACGGCACCACCTGCA | | Novagen | 20 | CBDcexLEAD | TAGGTGCAACTGTTGTTCTG | | Novagen | 21 | CBDclos | CAACACCAGTTGTAAATCCA | | Novagen | 22 | cIForward | GGATAGCGGTCAGGTGTT | pHybcI/HK | Invitrogen | 23 | Citeprimer | GGGGACGTGGTTTTCCTTTG | pCITE | Novagen | 24 | CMVForward | CGCAAATGGGCGGTAGGCGTG | Universal | | 25 | CYC1Reverse | GCGTGAATGTAAGCGTGAC | Cyc1 | Invitrogen | 26 | DsbA | CGAGTATGCTGATACAGTGA | DsRed | Clontech | 27 | DsbC | GAATTTCTCGACGAACACCA | DsRed | Clontech | 28 | DsRed1-C | AGCTGGACATCACCTCCCACAACG | DsRed | Clontech | 29 | DsRed1-N | GTACTGGAACTGGGGGGACAG | DsRed | Clontech | 30 | DsRed-C | AAGAAGCCTGTGCAGCTACCAGG | DsRed | Clontech | 31 | DsRed-N | CGCCTTCTATTTCAAACTCGTGCC | DsRed | Clontech | 32 | EBVReverse | GTGGTTTGTCCAAACTCATC | EBV | Invitrogen | 33 | EF-1aForward | TCAAGCCTCAGACAGTGGTTC | | | 34 | EGFP-C | CATGGTCCTGCTGGAGTTCGTG | EGFP | Clontech | 35 | EGFP-N | CGTCGCCGTCCAGCTCGACCAG | EGFP | Clontech | 36 | GAL1Forward | AATATACCTCTATACTTTAACGTC | Gal1 | Invitrogen | 37 | Glprimer1 | TGTATCTTATGGTACTGTAACTG | | | 38 | Glprimer2 | CTTTATGTTTTTGGCGTCTTCCA | | | 39 | gp64promoter | CTACTAGTAAATCAGTCACACC | | | 40 | gp64Signalprimer | GCGCTATTGTTTTATATGTGC | | | 41 | IE1promoterprimer | TGGATATTGTTTCAGTTGCAAG | pIEx,pBIEx | Novagen | 42 | Lamdagt10For | CTTTTGAGCAAGTTCAGGCCTGGTTAAG | LambdaGT10 | Promega | 43 | Lamdagt10Rev | GAGGTGGCTTATGAGTATTTCTTCCAGG | LambdaGT10 | Promega | 44 | Lamdagt11For | GGTGGCGACGACTCCTGGAGCCCG | LambdaGT11 | Promega | 45 | Lamdagt11Rev | TTGACACCAGACCAACTGGTAATG | LambdaGT11 | Promega | 46 | M13Forward(-20) | GTAAAACGACGGCCAG | Universal | | 47 | M13Forward(-40) | GTTTTCCCAGTCACGAC | Universal | | 47b | M13Reverse | CAGGAAACAGCTATGAC | Universal | | 48 | pUCR/M13Forward | CCCAGTCACGACGTTGTAAAACG | pUC | PGEM-T PGEM- | 49 | pUCR/M13Reverse | AGCGGATAACAATTTCACACAGGAA | pUC | PGEM-TPGEM- | 50 | MTForward | CATCTCAGTGCAACTAAA | pMT/V5-His | Invitrogen | 51 | myc-HisReverse | ATGACCGGTATGCATATTCAG | | | 52 | OpIE2Forward | CGCAACGATCTGGTAAACAC | pMIB/V5-His | Invitrogen | 53 | OpIE2Reverse | GACAATACAAACTAAGATTTAGTCAG | pMIB/V5-His | Invitrogen | 54 | p10Forward | GTATATTAATTAAAATACTATACTG | pTriEx-2Hygro | Novagen | 55 | pBADForward | ATGCCATAGCATTTTTATCC | E.coliaraBAD | Invitrogen | 56 | pBADReverse | GATTTAATCTGTATCAGG | E.coliaraBAD | Invitrogen | 57 | pCDM8Reverse | TAAGGTTCCTTCACAAAG | pCDM8 | Invitrogen | 58 | pCEPForward | AGAGCTCGTTTAGTGAACCG | | | 59 | pCMVForward | GATCCGGTACTAGAGGAACTGAAAAAC | | | 60 | pDABForward | ATGCCATAGCATTTTTATCC | | Invitrogen | 61 | pETUpstreamPrimer | ATGCGTCCGGCGTAGA(16) | | | 62 | pETBlueDOWNPrimer | GTTAAATTGCTAACGCAGTCA | | | 63 | pETBlueUPPrimer | TCACGACGTTGTAAAACGAC | | | 64 | pFastBacForward | GGATTATTCATACCGTCCCA | pFastBac | Invitrogen | 65 | pFastBacReverse | CAAATGTGGTATGGCTGATT | pFastBac | Invitrogen | 66 | pGAPForward | GTCCCTATTTCAATCAATTGAA | pGAPz | Invitrogen | 67 | pGENEForward | CTGCTATTCTGCTCAACCT | | | 68 | pGEX3' | GAGCTGCATGTGTCAGAGG | Universal | | 69 | pGEX5' | GGCAAGCCACGTTTGGTG | Universal | | 70 | pHookForward | ACGGTGCATTGGAACGGAC | pHook-2,-3 | Invitrogen | 71 | pHookReverse | GATTGCGTCGCATCGACCC | pHook-2,-3 | Invitrogen | 72 | pHybLex/ZeoForward | AGGGCTGGCGGTTGGGGTTATTCGC | pHybLex/Zeo | Invitrogen | 73 | pHybLex/ZeoReverse | GAGTCACTTTAAAATTTGTATACAC | pHybLex/Zeo | Invitrogen | 74 | PinPointSequencingprimer | CGTGACGCGGTGGAGGGCG | PinPointXa-1,-2,-3,PinPointXa-1T-vector | Promega | 75 | pJET1.2Forward | CGACTCACTATAGGGAGAGCGGC | | | 76 | pJET1.2Reverse | AAGAACATCGATTTTCCATGGCAG | | | 77 | pJG4-5Forward | GATGCCTCCTACCCTTATGATGTGCC | pJG4-5 | | 78 | pJG4-5Reverse | GGAGACTTGACCAAACCTCTGGCG | pJG4-5 | | 79 | PolyhedrinForward | AAATGATAACCATCTCGC | pVL1393 | Invitrogen | 80 | PolyhedrinReverse | GTCCAAGTTTCCCTG(15) | pVL1393 | Invitrogen | 81 | pQE-TriSystemForward | GTTATTGTGCTGTCTCATC | | | 82 | pQE-TriSystemReverse | TCGATCTCAGTGGTATTTGTG | | | 83 | pREPForward | GCTCGATACAATAAAGCCC | pREP4 | Invitrogen | 84 | pRset | CTAGTTATTGCTCAGCGGTGG | pRset | Invitrogen | 85 | pRHForward | CTGTCTCTATACTCCCCTATAG | pRH | Invitrogen | 86 | pRHReverse | CAAAATTCAATAGTTACTATCGC | pRH | Invitrogen | 87 | pRSETReverse | TAGTTATTGCTCAGCGGTGG | pRSET | Invitrogen | 88 | pTargetSequencingPrimer | TTACGCCAAGTTATTTAGGTGACA | pTarget | Promega | 89 | pTRCHisForward | GAGGTATATATTAATGTATCG | pTrcHis | Invitrogen | 90 | pTrcHisReverse | GATTTAATCTGTATCAGG | pTrcHis | Invitrogen | 91 | pTRE3' | CCACACCTCCCCCTGAAC | pTRE | BDBiosciences | 92 | pTRE5' | CGCCTGGAGACGCCATCC | pTRE | | 93 | pTriplEx3' | ACTCACTATAGGGCGAATTG | pTriplEx | Clontech | 94 | pTriplEx5' | CTCGGGAAGCGCGCCATTGTGTTGGT | pTriplEx | Clontech | 95 | pUniForward | CTATCAACAGGTTGAACTG | pUni | Invitrogen | 96 | pUniReverse | CAGTCGAGGCTGATAGCGAGCT | pUni | Invitrogen | 97 | pYESTrpForward | GATGTTAACGATACCAGCC | pYESTrp | Invitrogen | 98 | pYESTrpReverse | GCGTGAATGTAAGCGTGAC | pYESTrp | Invitrogen | 99 | QEPromoter | CCGAAAAGTGCCACCTG | pQE | Qiagen | 100 | QEReverse | GTTCTGAGGTCATTACTGG | pQE | Qiagen | 101 | R-20merPrimer | CAGCTATGACCATGATTACG | pSTBlue-1 | Novagen | 102 | RsaAReverse | GCCGCGCCAGCGACGCGGAGGG | pCX | Invitrogen | 103 | RVprimer3 | CTAGCAAAATAGGCTGTCCC | pGL/pCAT3 | Promega | 104 | Rvprimer4 | GACGATAGTCATGCCCCGCG | pGL/pCAT3 | Promega | 105 | SeqL-A(proximaltoattL1) | TCGCGTTAACGCTAGCATGGATCTC | pDONR201/pDONR207 | Invitrogen | 106 | SeqL-B(proximaltoattL2) | GTAACATCAGAGATTTTGAGACAC | pDONR201/pDONR207 | Invitrogen | 107 | STag18merPrimer | GAACGCCAGCACATGGAC | pIEx-1 | Novagen | 108 | STagPrimer | CGAACGCCAGCACATGGACA | pIEx-1 | Novagen | 109 | Sp6Promoter | GATTTAGGTGACACTATAG | Universal | | 110 | SV40-pArev | CCTCTACAAATGTGGTATGG | pRL-SV40 | | 111 | SV40-Promoter | GCCCCTAACTCCGCCCATCC | pRL-SV40 | | 112 | T3Promoter | ATTAACCCTCACTAAAGGGA | Universal | | 113 | T7EEV | ATGTCGTAATAACCCCGCCCCG | pAlterMAX,pSI,pCI,pCI-Neo,pCMVTnT,pTnT,phMGFPVector,HaloTagpHT2,psiCHECK-1,-2 | Promega | 114 | T7gene10Primer | TGAGGTTGTAGAAGTTCCG | | | 115 | T7Promoter | TAATACGACTCACTATAGGG | Universal | | 116 | T7Reverse | TAGTTATTGCTCAGCGGTGG | Universal | | 117 | T7Terminator | GCTAGTTATTGCTCAGCGG | Universal | | 118 | U6Primer | GGGCAGGAAGAGGGCCTAT | | | 119 | U-19merPrimer | GTTTTCCCAGTCACGACGT | M13mp18,pCITE | Novagen | 121 | V5ReversePrimer | ACCGAGGAGAGGGTTAGGGAT | V5Epitope | Invitrogen | 122 | VP22Forward | GGCCACGGCGACTCGA | | | 123 | XpressForward | TATGGCTAGCATGACTGGT | XpressEpitope | Invitrogen | 124 | VLH | TTGTGTGGAATTGTGAGCGG | | | 125 | VF2 | TGCCACCTGACGTCTAAGAA | | | 126 | VR | ATTACCGCCTTTGAGTGAGC | | | 127 | TKpARev | CTTCCGTGTTTCAGTTAGC | | | 128 | pQE60-FW | CCCGAAAAGTGCCACCTG | | | 129 | pQE60-Rv | GTTCTGAGGTCATTACTGG | | | 130 | SeqL-A(ATTL1) | GCGAGAGTAGGGAACTGC | pENTR | Invitrogen | 131 | SeqL-B(ATTL2) | AACATCAGAGATTTTGAGACAC | pENTR | Invitrogen | 132 | pmirGloF(7205bp) | GAGGTGCCTAAAGGACTGAC | pmirGlo | promega |
|