Description: RecombinantProteinGisanimmunoglobulin-bindingproteinderivedfromthecellwallofcertainstrainsofbeta-hemolyticStreptococci.ItbindswithhighaffinitytotheFcportionofvariousclassesandsubclassesofimmunoglobulinsfromavarietyofspecies.ThealbuminandcellsurfacebindingdomainshavebeeneliminatedfromRecombinantProteinGtoreducenonspecificbindingand,therefore,canbeusedtoseparateIgGfromcrudesamples.DuetoitsaffinityfortheFcregionofmanymammalianimmunoglobulins,proteinGisamajorapplicationinpurifyingantibodies.ProteinGisconsideredauniversalreagentinbiochemistryandimmunology. Applications: - Antibodypurification
- Proteinpurification
- Isolatesimmunecomplexes
- Immunoprecipitation
- ImmunoaffinityPurification
- WesternBlotting
Source: PurifiedfromE.colithatcarriestheproteinGgeneofGStreptococci. Purity: >98%bySDS-PAGEandHPLCanalyses.ThealbuminbindingdomainaswellascellwallandcellmembranebindingdomainshavebeenremovedtoensurethemaximumspecificIgGbindingcapacity.ThisproteincontainsonlyIgGbindingdomains. RecommendedStorageCondition: -20ºC.Avoidfreeze/thawcycles. Usage: Underoptimalconditions,1mgproteinGwillbindapproximately5mghumanIgG.OptimalbindingofProteinGtoantibodiesoccursatpH5.0to6.0andcanbeelutedoverapHrangeof2.5to3.0. |