Description: Theβ-galactosidasesubstrateX-Gal(5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside)isachromogenicstainforβ-galactivity,andiscommonlyusedtodistinguishbetweenrecombinantsandnonrecombinantsbylacZα-complementationwithappropriatevectorsandhosts.X-GalSolutionisprovidedasaconvenient20mg/mlconcentrateinDMSO,readyfordilutiontoculturemediumorappropriatebuffers.
Synonym:5-Bromo-4-chloro-3-indolylβ-D-galactoside,BCIG,X-Gal
Applications: -Blue/whitecolonyscreeningassay(blue:non-recombinantcolonies&white:recombinantcolonies). -X-Galstainingofeukaryoticcellsforthedetectionofβ-galactosidasereportergeneexpression. -Detectionofβ-galactosidaseactivityinvariousimmunologicalandhistochemicalapplications.
Formula:C14H15BrClNO6
Molecularweight:408.6
Note: -Petridish(9cmdiameter):Spread40µLonthesurface -LiquidLBagar:Use2µLforeach1mLofliquidLBagar,addjustbeforepouringplates(atabout50°C) -Transfectedeukaryoticcells:use25µL(1mg)foreach1mLofstainingsolution
RecommendedStorageCondition:Stableupto6monthswhenstoredandprotectedfromlightat4°C.
Reference: 1.J.Sambrook,D.W.Russell,MolecularCloning:ALaboratoryManual(ColdSpringHarborLaboratoryPress,ColdSpringHarbor,NewYork,ed.3,2001),pp.1.124-1.125,A1.27. |